Coding

Part:BBa_K571000:Design

Designed by: Lu-Chu Ke   Group: iGEM11_TzuChiU_Formosa   (2011-10-02)


acsAB/pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1981
    Illegal PstI site found at 736
    Illegal PstI site found at 1132
    Illegal PstI site found at 1972
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1981
    Illegal PstI site found at 736
    Illegal PstI site found at 1132
    Illegal PstI site found at 1972
    Illegal NotI site found at 3147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1981
    Illegal BglII site found at 1136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1981
    Illegal PstI site found at 736
    Illegal PstI site found at 1132
    Illegal PstI site found at 1972
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1981
    Illegal PstI site found at 736
    Illegal PstI site found at 1132
    Illegal PstI site found at 1972
    Illegal NgoMIV site found at 2355
    Illegal NgoMIV site found at 2395
    Illegal AgeI site found at 4527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1069


Design Notes

By comparative, acsAB is long base pairs gene, so we need more primer during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results.


Source

  • organism: Gluconacetobacter hansenii ATCC 23769
  • GenBank: AB091060.1

References